Real Time PCR NRTP-500 supports a 96-well sample capacity for efficient high-throughput testing. It accommodates reaction volumes ranging from 10 to 50μl for flexible assay setup. This unit uses an electronic, automatic heated lid to ensure consistent and secure sample sealing. It achieves fast heating rates of up to 6°C/s for rapid thermal cycling. Our PCR system enables multiplex detection of five fluorescent dyes in a single well for detailed data collection.
| Sample capacity | 96 wells |
| Reaction volume | 10 to 50μl |
| Heating/cooling method | Peltier |
| Maximum ramp rate | 6℃/s |
| Temperature setting range | 4 to 100℃ |
| Temperature accuracy | ±0.2℃ |
| Temperature uniformity | ±0.2℃ |
| Gradient zone | 12 columns |
| Gradient range | 1 to 36℃ |
| Heated lid | Electronic automatic lid |
| Excitation source | Long-life, high-performance LEDs |
| Detector | Highly sensitive MPPC with a Fresnel lens |
| Scanning principle | Time-resolved scanning technology |
| Detector position | Top of the block |
| Excitation/detection range | 455 to 650nm/510 to 715nm |
| Fluorescence channel | 4 |
| Detection sensitivity | 1 copy of the target sequence |
| System sensitivity | Detect differences as small as 1.33-fold in target quantities in singleplex reactions |
| Dynamic range | 10 orders of magnitude |
| Dye compatibility | FAM/SYBR Green, VIC/JOE/HEX/TET, NED/TAMRA/Cy3 JUN, ROX/Texas Red, Mustang Purple, Cy5/LIZ |
| Data analysis modes | Absolute quantification, Relative quantification, Endpoint qualitative analysis, Melt curve analysis, Protein Stability Screening, Genotyping |
Real Time PCR NRTP-500 is used for accurate detection, quantification, and analysis of genetic material, enabling precise monitoring of gene expression, mutations, and pathogens. It is commonly applied in research laboratories, clinical diagnostics, agricultural biotechnology, and pharmaceutical development.
